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Fourth Semester M.Sc. Degree Examination, May 2020BotanyBO 242 a - BIOTECHNOLOGY(2013 Admission Onwards)


J-4882
Reg. No......
 Name :.........
Fourth Semester M.Sc. Degree Examination, May 2020
Botany
BO 242 a - BIOTECHNOLOGY
(2013 Admission Onwards)


Max. Marks: 75

I.Answer the following questions


1.Define Palindromic sequences.
2.What is meant by Probiotics?
3.Explain the terms YAC and BAC.
4. Define Klenow fragment.
5.What is meant by Bio piracy?
6. Give the speciality of ddNTPs.
7. Briefly explain Somatostatin.
8. What are the advantages of Filter sterilization?
9. Give the significance of Star activity.
10. Give significance of Redifferentiation.

(10 x 1 = 10 Marks)

II. Answer the following questions in not more than 50 words

11. (a) Differentiate Taq DNA polymerase and DNA polymerase 1 used in thermal
cycler.
OR
(b) Explain any one hybridization based molecular marker.


12. (a) Differentiate RFLP and RAPD.
OR
(b) Explain 2D-electrophoresis.

13. (a) Give a note on Somatic embryogenesis.
OR
(b) Explain the role of protoplast culture in cytoplasmic male sterility.

14. (a) What modification was made to DNA Polymerase 1 using in PCR and why?
OR
(b) Explain Ter sites and their importance.
15. (a) Differentiate silent mutation and neutral mutation.
OR
(b) Briefly explain liposome mediated gene transfer.

(5 x 2 = 10 Marks)
III. Answer the following questions in not more than 150 words.
16. (a) Explain cloning vectors and expression vectors.
OR
(b) Explain the role of transposons in gene targeting.

17. (a) Give a note on strategy employed in immobilization of enzymes and cells.
OR

(b) Explain thermo cycler and its application.

18. (a) Explain chemical degradation method for DNA sequencing.
OR
(b) Give an account on vectors used in genetic engineering.

19. (a) Explain Gameto-clonal variations in crop improvement.
OR
(b) Write a note on SCAR, STR, SSR molecular markers.

20. (a) Explain Intellectual Property Right-IPR.
OR
(b) Comment on social and ethical issues of biotechnology


21. (a) Role of DNA methylation and histone modification in manipulation of gene expression in prokaryotes.
OR
(b) Give a brief account on Agrobacterium mediated transformation.

22. (a) Explain Monoclonal antibodies and its application.
OR
(b) Write a note on different types of bioreactors.

(7 x 5 = 35 Marks)

IV. Answer the following questions in not more than 250 words

23. (a) Elaborate on the production, purification and applications of microbial enzymes and organic compounds?
OR
(b) Give a detailed account on types of enzymes used in recombinant DNA technology.

24. (a) Explain in detail about terminator technology and the pros and cons associated with it.
OR
(b) Explain in detail about objectives and achievements of genetically modified
organisms.

(2 x 10 = 20 Marks)

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