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Fourth Semester M.Sc. Degree Examination, June 2022BotanySpecial Paper II - Elective BO 242 a BIOTECHNOLOGY


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Fourth Semester M.Sc. Degree Examination, June 2022
Botany
Special Paper II - Elective
BO 242 a BIOTECHNOLOGY
-
(2013-2018 Admission)

Time: 3 Hours
Max. Marks 75
Give Illustrations wherever necessary.
1.Answer the following questions.

1.Define a calliclone.
2.Which fungi helps in fermentation?
3.How is hairy root culture induced in plants?
4.Name a microbe that produces organic acid.
5.What is the advantage of chromosome elimination technique?
6.Describe the significance of PIPS.
7.What is elicitation in plant tissue culture?
8.Describe the term bioconversion.
9.Which is the most successful chemical used for protoplast fusion?
10. Define cybridization.

(10x1 =10 Marks)

II.Answer the following questions in not more than 50 words.


11. (a) Give an account on signature tagged mutagenesis.
OR
(b) Write notes on cytoplasts and their importance?

12. (a) Give an account of gene theft.
OR
(b) Describe induced androgenesis and gynogenesis for haploid plant
production.

13. (a) Distinguish between blue and brown biotechnology.
OR
(b) Why are mutations more common in prokaryotes?

14. (a) Describe suspension culture.
OR
(b) Describe the common enzymes used in genetic engineering

15. (a) Explain the details of IPR and patenting.
OR
(b) What are the benefits of taking probiotics?
III. Answer the following questions in not more than 150 words.

16. (a) Explain inducible and repressible operons.
OR
(b) Compare DNA fingerprinting and DNA footprinting.

17. (a) Discuss the steps involved in somatic hybridization.
OR
(b) Describe transposon mediated gene tagging.
18. (a) Describe the similarities and differences between upstream and
downstream bioprocessing.
OR
(b) Explain membrane technology and it's applications.
19. (a) Describe the Maxam-Gilbert method of sequencing.
OR
(b) Discuss on random amplification of polymorphic DNA.
20. (a) Describe the genetic vectors used in recombinant DNA technology.
OR
(b) Give an account of the different types of bioreactors.
21. (a) Discuss the various consequences of genetically modified organisms.
OR
(b) Give an account of the salient features and applications of petro-plants.
22. (a) Describe the features of gene delivery and it's uses.
OR
(b) Differentiate c-DNA from genomic DNA.


(7x5 35 Marks)

IV. Answer the following questions in not more than 250 words.

23. (a) Discuss on the different types of blotting techniques. Add notes on dot blots.
OR
(b) Give an account on transgenic and cisgenic organisms.
24. (a) Describe the components of culture media used in fermentation Mention the factors that influence microbial growth.
OR
(b) Microorganisms that are used for industrial production must meet certain requirements. Discuss.
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(2 x 10 = 20 Marks)

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