Skip to main content

Third Semester M.Sc. Degree Examination, March 2025BotanyBO 232: BIOCHEMISTRY, PLANT PHYSIOLOGY AND RESEARCHMETHODOLOGY


Third Semester M.Sc. Degree Examination, March 2025
Botany
BO 232: BIOCHEMISTRY, PLANT PHYSIOLOGY AND RESEARCH
METHODOLOGY

Time: 3 Hours
(2019 Admission Onwards)
Max. Marks: 75


(Instruction: Draw diagrams and illustrate with examples wherever necessary)
1.Answer the following questions.


1.What is Km value? Mention its significance.
2.Draw the structure of sucrose.
3.List any two-sulphur containing amino acids.
4.What is salinity stress?
5.Name any two weedicides.
6.What is lag phase?
7.What are phytoalexins?
8.What are quantasomes?
9.What is biochemical methodology of experimental design?
10. List any two statistical tools for the citation of reference.
(10 × 1 = 10 Marks)


II.Answer the following questions not more than 50 words.

11. (a) Compare pH and pKa? Mention its significance.
                      OR
(b) What is monoacyl glycerol? Explain its biosynthesis.

12. (a) Explain the classification of protein based on function.
                        OR
(b) Compare secondary and quaternary structure of proteins.


13. (a) What is a biological clock? How does it differ from water oxidizing clock?
                   OR
(b) How does the breakdown of fatty acids occur during seed germination?


14. (a) Compare symbiotic and nonsymbiotic nitrogen fixation in plants.
                 OR
(b) Compare phloem loading and phloem unloading.


15. (a) What are the essential tips for preparing the methodology section of a research paper?
               OR
(b) Explain the importance of the abstract and title in a thesis.
(5 x 2 = 10 Marks)

III. Answer the following questions not more than 150 words.

16. (a) What are abzymes? Give an example.
                    OR
(b) Explain mevalonic acid pathway of terpenoids.




17. (a) What are protein domains? Mention it importance in enzyme activity.
OR
(b) Describe the oxidation of lipids. Mention different types of it.

18. (a) Briefly describe the structure and biosynthesis of adenine.
OR
(b) What is RUBISCO? Mention its importance.


19. (a) Give a concise account on cold stress and its physiologic response in plants.
OR
(b) Explain the various physiological effects of ethylene.


20. (a) Draw the schematic representation of glycolate metabolism.
OR
(b) Explain the physiology of abscission.


21. (a) Write an account on the structure and functions of phytochromes.
OR
(b) What is allelopathy? Mention its importance in plants with examples.

22. (a) Write an account on various steps of a research design.
OR
(b) Write a note on the use of statistical tools and the interpretation of results with any statistical tool you have studied.
3
(7 x 5 = 35 Marks)


III. Answer the following questions in not more than 250 words.

23. (a)Explain the structure, function and metabolism of starch.
OR
(b) Explain IUB system of classification and nomenclature of enzymes. Add a note on its regulation of enzyme activity.


24. (a) Create a schematic representation of the C3 pathway and provide an explanation. Why are CAM plants called so?
          OR
(b) Critically analyze aerobic and anaerobic respiration in plants. Add a note on the regulation of the TCA cycle.
(2 x 10 = 20 Marks)




Comments

Popular Posts

❥NORTHERN BLOTTING

NORTHERN BLOTTING – 30 MARK DETAILED NOTES  𓆞❥ 𓆞❥ 𓆞❥ 𓆞❥ 𓆞❥ 𓆞 ❥ 𓆞❥ 𓆞❥  Northern blotting is a molecular biology technique used to detect specific RNA molecules in a complex mixture. It provides information about gene expression, RNA size, and transcript abundance by hybridizing RNA with a labeled complementary DNA or RNA probe. 📌 Named by analogy to Southern blotting (DNA detection). 2. Principle The principle of Northern blotting is based on: Separation of RNA molecules by size using denaturing agarose gel electrophoresis Transfer (blotting) of separated RNA onto a nylon or nitrocellulose membrane Hybridization of membrane-bound RNA with a labeled complementary probe Detection of RNA–probe hybrids by autoradiography or chemiluminescence ✔ Only RNA sequences complementary to the probe will be detected. 3. Types of RNA Analyzed mRNA (most common) rRNA tRNA miRNA and siRNA (with modified protocols) 4. Requirements / Materials Total RNA or poly(A)+ RNA Denaturing agarose ...

Biological Databases – Types of Data and DatabasesNucleotide Sequence Databases (EMBL, GenBank, DDBJ)

Biological Databases – Types of Data and Databases Nucleotide Sequence Databases (EMBL, GenBank, DDBJ) 1. Introduction Biological databases are systematic, computerized collections of biological information that allow efficient storage, retrieval, updating, and analysis of large volumes of biological data. With the advent of genome sequencing, molecular biology, and bioinformatics, biological databases have become essential tools in biological research. These databases support studies in genomics, proteomics, evolutionary biology, taxonomy, medicine, agriculture, and biotechnology. 2. Types of Data Stored in Biological Databases Biological databases store diverse types of biological information, including: 1. Sequence Data DNA sequences RNA sequences Protein sequences 2. Structural Data Three-dimensional structures of proteins Nucleic acid structures 3. Functional Data Gene functions Enzyme activity Regulatory elements 4. Genomic Annotation Data Gene location Exons, introns Promoters a...

Information retrieval from databases - search concepts, Tools for searching, homology searching, finding Domain and Functional site homologies

Information retrieval from databases - search concepts, Tools for searching, homology searching, finding Domain and Functional site homologies Information Retrieval from Databases 1. Introduction Information retrieval in bioinformatics refers to the process of extracting relevant biological data (DNA, RNA, protein sequences, structures, or functional information) from databases. Aim : Identify sequences, functions, or structural features for analysis, comparison, and annotation. Databases can be primary (raw sequence data) or secondary/derived (annotated, processed data). 2. Search Concepts in Biological Databases 2.1 Types of Searches Exact Match Search Returns results only if the query exactly matches database entries. Useful for known accession numbers or IDs. Pattern/Keyword Search Searches based on specific motifs, keywords, or annotations. Example: “kinase domain,” “signal peptide.” Similarity/Homology Search Detects sequences similar to the query based on sequence alignment. Use...

❃HPLC – High Performance Liquid Chromatography

HPLC – High Performance Liquid Chromatography ┏━━━━━ •❃°•°❀°•°❃•━━━━•━━━┓  1. Introduction High Performance Liquid Chromatography (HPLC) is an advanced analytical technique used for the separation, identification, and quantification of components present in a mixture. It is based on the differential distribution of analytes between a stationary phase and a liquid mobile phase under high pressure. HPLC is widely used in biochemistry, biotechnology, pharmaceuticals, food analysis, environmental studies, and clinical diagnostics. 2. Principle of HPLC The principle of HPLC is based on partition, adsorption, ion-exchange, or size-exclusion mechanisms, depending on the type of column used. A liquid mobile phase is pumped at high pressure through a column packed with fine stationary phase particles Sample components interact differently with the stationary phase Components with stronger interaction elute slower Components with weaker interaction elute faster Separated components are detec...

Exploitation of Somaclonal and Gametoclonal Variations for Plant Improvement

Exploitation of Somaclonal and Gametoclonal Variations for Plant Improvement  1. Introduction Plant tissue culture often induces genetic and epigenetic variations among regenerated plants. These variations, when stable and heritable, can be exploited as a source of novel traits for crop improvement. Somaclonal variation: Variation arising in plants regenerated from somatic cells cultured in vitro. Gametoclonal variation: Variation arising in plants regenerated from gametic cells (anther, pollen, ovule culture). Both provide additional genetic variability beyond conventional breeding. 2. Somaclonal Variation 2.1 Definition Somaclonal variation refers to genetic variation observed among plants regenerated from somatic tissue cultures, such as callus, suspension cultures, or explants. Term coined by Larkin and Scowcroft (1981). 2.2 Sources of Somaclonal Variation Chromosomal changes Aneuploidy Polyploidy Chromosome rearrangements Gene mutations Point mutations Insertions and deletions...

Microbial Production of PharmaceuticalsSomatostatin, Humulin and Interferons

Microbial Production of Pharmaceuticals Somatostatin, Humulin and Interferons 1. Introduction Advances in recombinant DNA technology have enabled microorganisms to produce human therapeutic proteins safely, economically and in large quantities. Microbial systems such as Escherichia coli and yeast (Saccharomyces cerevisiae) are widely used for the production of pharmaceuticals that were earlier isolated from human or animal tissues. Important microbial-derived pharmaceuticals include somatostatin, human insulin (Humulin) and interferons. 2. Advantages of Microbial Production of Pharmaceuticals High yield and rapid production Cost-effective and scalable Free from animal pathogens Consistent product quality Easy genetic manipulation 3. General Steps in Microbial Production of Recombinant Pharmaceuticals Isolation of target gene Construction of recombinant DNA Insertion into suitable vector Transformation into host microorganism Expression of protein Downstream processing and purification ...

𓆉 INDEX PAGE -NOTETHEPOINT43

INDEX PAGE   MAIN    CONTENT 1.   HSST BOTANY SYLLABUS, DETAILED NOTES, MCQ 2.  SET GENERAL PAPER SYLLABUS, DETAILED NOTES, 50MCQ 3.  SET BOTANY SYLLABUS, DETAILED NOTES, MCQ 4. MSC BOTANY THIRD SEMESTER SYLLABUS, NOTES (KERALA UNIVERSITY ) 5. MSC BOTANY THIRD SEMESTER QUESTION PAPER (KERALA UNIVERSITY ) 6. MSC BOTANY FOURTH SEMESTER SYLLABUS &NOTES (KERALA UNIVERSITY ) 7. FOURTH SEMESTER MSC BOTANY PREVIOUS QUESTION PAPER  (KERALA UNIVERSITY )