Skip to main content

Fourth Semester M.Sc. Degree Examination, September 2019BotanySpecial Paper II - ElectiveBO 242 a: BIOTECHNOLOGY(2013 Admission onwards)

Reg. No....... 
Name.........

G-5263
Fourth Semester M.Sc. Degree Examination, September 2019
Botany
Special Paper II - Elective
BO 242 a: BIOTECHNOLOGY
(2013 Admission onwards)

Max. Marks: 75


1.Answer the following questions:
1.Humulin
2.YAC
3. Cybrids
4.Hybridomas
5.IPR
6.Gene therapy
7.C DNA library
8.AFLP
9. Hairy root culture
10. Somacional variation
(10 x 1=10 Marks)

II.Answer the following questions in not more than 50 words:

11. (a) What are immobilized enzymes? What is its advantage?
OR
(b) Write a short note on molecular farming.

12. (a) Give an account of bioprocess technology for the production of secondary metabolites.
OR
(b) What are bioreactors? How it operates?

13. (a) What are probiotics?. How do they work?
OR
(b) Discuss the methodology and application of western blotting.


14. (a) Briefly explain the application of protoplast culture
OR
(b) Write a short note on gene therapy


15. (a) What are reporter genes? Discuss its utility in transformation studies
OR
(b) What are cosmids ? What is its application.

(5 x 2 = 10 Marks)
III. Answer the following questions in not more than 150 words:

16. (a) Explain PCR and its different forms with significance.
OR

(b) Explain different types of restriction enzymes and target sites.

17. (a) Explain DVA finger printing and its application.
OR
(b) Explain the technique and application of callus culture.

18. (a) Explain the role of linkers, adaptors and homopolymers in the generation of recombinant DNA.
OR
(b) Discuss the role of Agrobacterium and its plasmid in the transformation of plants.

19. (a) Explain the microbial production and purification of industrial enzymes and organic compounds.
OR
(b) Explain fermentation technology and its application.

20. (a) Explain the impact of biotechnology on agri.- biodiversity, medicine and
environment.
OR
(b) What is genomic DNA library? Discuss the details about the construction of genomic library.
21. (a) What is cell suspension culture? Explain the kinetics of growth and elicitation methods related to suspension culture.
OR
(b) What is bioremediation? Give an account of role of transgenic microbes in bioremediation.
22. (a) Explain gene targeting and its application
OR
(b) Explain site directed mutagenesis with its significance.

(7 x 5 = 35 Marks)


IV. Answer the following questions in not more than 250 words:

23. (a) Explain different types of gene transfer technologies and its application.
OR
(b) Explain the replication of bacterial DNA and regulation of gene expression

24. (a) Explain the application of transgenic technology in plants
OR
(b) Explain about the application of plant tissue culture technology for the large scale production of crop plants.


(2 x 10 = 20 Marks)




Comments

Post a Comment

Popular Posts

Fourth Semester M.Sc. Degree Examination, June 2022BotanySpecial Paper II - ElectiveBO 242 a: BIOTECHNOLOGY

Reg. No.: Name: N-6273 Fourth Semester M.Sc. Degree Examination, June 2022 Botany Special Paper II - Elective BO 242 a: BIOTECHNOLOGY Time: 3 Hours (2019 Admission Onwards) Max. Marks: 75 1. Instruction: Draw diagrams and illustrate with examples wherever necessary. Answer the following questions. 1. What are the desirable features of a cloning vehicle? 2. What is a palindrome? 3. What is the significance of Ori C site? 4. What is the actual function of restriction enzymes in a bacterial system?  5.Name any two bacteria and fungi used for alcohol fermentation. 6. What is a starter culture? 7. What are adapters? 8. What are probes? 9. What is biopiracy? 10. Define cybrids. (10 x 1 = 10 Marks) II. Answer the following questions in not more than 50 words .  11. (a) Why is callus culture a prerequisite for somaclonal variations? OR (b) How is virus elimination done via plant tissue culture? 12. (a) How is aeration maintained in a bioreactor? OR (b) What are the methods available f...
Post a Comment
Read more

Electroporation – Detailed Notes

Electroporation – Detailed Notes Definition : Electroporation is a physical method of gene transfer in which cells are exposed to a brief, high-voltage electric pulse, creating temporary pores in the cell membrane. This allows DNA, RNA, proteins, or other molecules to enter the cytoplasm. It is widely used in bacteria, yeast, plant protoplasts, and mammalian cells. Key Concept: The electric field destabilizes the membrane, making it permeable to macromolecules. 1. Principle Cells are suspended in a conductive medium. A brief electrical pulse induces transient pores in the plasma membrane. DNA or other molecules present in the medium enter the cell through these pores. Membrane reseals after the pulse, and the molecule is retained inside the cell. Advantages of Principle: Direct and rapid. Works in many cell types. Does not require chemical carriers or viral vectors. 2. Materials Required Cells – bacterial, yeast, plant protoplasts, mammalian cells. DNA/RNA/other macromolecule – purifie...
Post a Comment
Read more

❃LC-MS (LIQUID CHROMATOGRAPHY – MASS SPECTROMETRY)

LC-MS (LIQUID CHROMATOGRAPHY – MASS SPECTROMETRY)  ┏━━━━━ •❃°•°❀°•°❃•━━━━•━━━┓ 1. INTRODUCTION LC-MS is a hyphenated analytical technique combining Liquid Chromatography (LC) and Mass Spectrometry (MS). It is used for separation, identification, and quantification of compounds in complex mixtures. LC separates analytes based on polarity, size, or charge, while MS detects molecules based on mass-to-charge ratio (m/z). Developed in the 1970s–1980s, LC-MS is now widely used in pharmaceutical, clinical, environmental, and food analysis. Importance : Detects trace levels of compounds (ng–pg range) Analyzes non-volatile, thermally labile compounds that cannot be analyzed by GC-MS Provides structural information through mass fragmentation Example: Detection of drugs in plasma, protein identification in proteomics, pesticide residue analysis in food. 2. COMPONENTS OF LC-MS The LC-MS system has three main parts: A. Liquid Chromatograph (LC) Function: Separates components of a mixture befor...
Post a Comment
Read more

Secondary Databases (PROSITE, PRINTS, BLOCKS)

Secondary Databases (PROSITE, PRINTS, BLOCKS  Secondary Databases Introduction Biological databases are broadly classified into primary and secondary databases. Primary databases store raw experimental data (e.g., nucleotide or protein sequences), whereas secondary databases contain derived information obtained by analyzing primary sequence data. Secondary databases are mainly used to: Identify protein families Detect conserved motifs, patterns, and domains Predict protein function Study structure–function relationships Examples of secondary databases include PROSITE, PRINTS, BLOCKS, Pfam, etc. 1. PROSITE Database Definition PROSITE is a secondary database that documents protein domains, families, and functional sites in the form of patterns and profiles. Developed by Swiss Institute of Bioinformatics (SIB) Maintained along with UniProt Principle PROSITE is based on the idea that functionally important regions of proteins are conserved during evolution. These conserved regions can ...
Post a Comment
Read more

𓆉 INDEX PAGE -NOTETHEPOINT43

INDEX PAGE   MAIN    CONTENT 1.   HSST BOTANY SYLLABUS, DETAILED NOTES, MCQ 2.  SET GENERAL PAPER SYLLABUS, DETAILED NOTES, 50MCQ 3.  SET BOTANY SYLLABUS, DETAILED NOTES, MCQ 4. MSC BOTANY THIRD SEMESTER SYLLABUS, NOTES (KERALA UNIVERSITY ) 5. MSC BOTANY THIRD SEMESTER QUESTION PAPER (KERALA UNIVERSITY ) 6. MSC BOTANY FOURTH SEMESTER SYLLABUS &NOTES (KERALA UNIVERSITY ) 7. FOURTH SEMESTER MSC BOTANY PREVIOUS QUESTION PAPER  (KERALA UNIVERSITY )
Post a Comment
Read more

Third Semester M.Sc. Degree Examination, December 2025BotanyBO 531: PLANT BREEDING, HORTICULTURE AND BIOSTATISTICS.

Third Semester M.Sc. Degree Examination, December 2025 Botany BO 531: PLANT BREEDING, HORTICULTURE AND BIOSTATISTICS (2024 Admission) Time: 3 Hours Max. Marks: 75 Answer these questions in one or two sentences.  Each question carries 1 mark. 1. Who introduced maize in India? 2.Name an organization in India for plant introduction. 3.  What is BSI? 4.What is Super Rice? 5.Define somaticplastic sterility? 6.What is a chemical mutagen? Give example. 7.What is Arboriculture? 8.What is MAP in Horticulture? 9.Define probability. 10. What is LSD in Biostatistics? (10 × 1 = 10 Marks) II.Answer the following questions in not more than 50 words . 11] Comment on Primary plant introduction.                OR 12. What are microcenters? Explain. 13.Explain zygotic sterility. How can we overcome this in plant breeding?                          OR 14 Describe a green house and its uses. ...
Post a Comment
Read more

Third Semester M.Sc. Degree Examination, February 2024 231: PLANT BREEDING, HORTICULTURE AND BIOSTATISTICS

Third Semester M.Sc. Degree Examination, February 2024                 Botany BO 231: PLANT BREEDING, HORTICULTURE AND BIOSTATISTICS (2019 Admission onwards) Time: 3 Hours I.Answer the following questions. 1.What is atomic gardening? 2.Name the cardamom research institute in Kerala. 3.Explain advantages of distant hybridisation. 4.Describe plant variety rights. 5.Write short notes on arboriculture. 6.What is vermicomposting? 7.Give short notes on cut flower industry. 8.What is ANOVA? 9.Describe the properties of binomial distribution. 10. Explain the use of LSD. Max. Marks: 75 (10 x 1 = 10 Marks) II.Answer the following questions in not more that 50 words. 11. (a) What do you mean by genetic modification techniques? OR (b) What is center of diversity of a species? 12. (a) Compare auto and allopolyploidy. OR (b) What are requirements of back cross breeding? 13. (a) Describe ideotype breeding and its significance. OR (b) What is the role of seed cer...
Post a Comment
Read more

Biological Databases – Types of Data and DatabasesNucleotide Sequence Databases (EMBL, GenBank, DDBJ)

Biological Databases – Types of Data and Databases Nucleotide Sequence Databases (EMBL, GenBank, DDBJ) 1. Introduction Biological databases are systematic, computerized collections of biological information that allow efficient storage, retrieval, updating, and analysis of large volumes of biological data. With the advent of genome sequencing, molecular biology, and bioinformatics, biological databases have become essential tools in biological research. These databases support studies in genomics, proteomics, evolutionary biology, taxonomy, medicine, agriculture, and biotechnology. 2. Types of Data Stored in Biological Databases Biological databases store diverse types of biological information, including: 1. Sequence Data DNA sequences RNA sequences Protein sequences 2. Structural Data Three-dimensional structures of proteins Nucleic acid structures 3. Functional Data Gene functions Enzyme activity Regulatory elements 4. Genomic Annotation Data Gene location Exons, introns Promoters a...
Post a Comment
Read more

Protein Structure Database (PDB)

Protein Structure Database (PDB) Introduction The Protein Structure Database (PDB) is the primary global repository for the three-dimensional (3D) structures of biological macromolecules such as proteins, nucleic acids, and protein–ligand complexes. These structures are determined experimentally using techniques like X-ray crystallography, Nuclear Magnetic Resonance (NMR) spectroscopy, and Cryo-Electron Microscopy (Cryo-EM). PDB plays a vital role in understanding: Protein structure and function Molecular interactions Drug discovery and design Structural biology and bioinformatics History and Development Established in 1971 Founded by Brookhaven National Laboratory (USA) Initially contained only 7 protein structures Now maintained by the Worldwide Protein Data Bank (wwPDB) Members of wwPDB RCSB PDB (USA) PDBe (Europe) PDBj (Japan) BMRB (Biological Magnetic Resonance Data Bank) Objectives of PDB To collect, store, and distribute 3D structural data of biomolecules To provide free and ope...
Post a Comment
Read more

Genetically modified microbes - biodegradation, biopesticides, bioremediation, mineral leaching and biofertilizers.

 Genetically Modified Microbes (GMMs) covering biodegradation, biopesticides, bioremediation, mineral leaching and biofertilizers.  Genetically Modified Microbes (GMMs) Introduction Genetically Modified Microbes (GMMs) are microorganisms such as bacteria, fungi, yeast or algae whose genetic material has been altered using recombinant DNA technology to enhance or introduce desirable traits. These microbes are engineered to improve efficiency, specificity and speed of biological processes useful in agriculture, industry and environmental management. GMMs play a vital role in sustainable development by reducing dependence on chemical fertilizers, pesticides and polluting industrial processes. 1. Genetically Modified Microbes in Biodegradation Definition Biodegradation is the microbial breakdown of complex organic pollutants into simpler, non-toxic substances. Role of GMMs Natural microbes often degrade pollutants slowly. Genetic modification enhances: Enzyme activity Substrate sp...
Post a Comment
Read more