Fourth Semester M.Sc. Degree Examination, September 2019BotanySpecial Paper II - ElectiveBO 242 a: BIOTECHNOLOGY(2013 Admission onwards)

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G-5263

Fourth Semester M.Sc. Degree Examination, September 2019

Botany

Special Paper II - Elective

BO 242 a: BIOTECHNOLOGY

(2013 Admission onwards)

Max. Marks: 75

1.Answer the following questions:

1.Humulin

2.YAC

3. Cybrids

4.Hybridomas

5.IPR

6.Gene therapy

7.C DNA library

8.AFLP

9. Hairy root culture

10. Somacional variation

(10 x 1=10 Marks)

II.Answer the following questions in not more than 50 words:

11. (a) What are immobilized enzymes? What is its advantage?

(b) Write a short note on molecular farming.

12. (a) Give an account of bioprocess technology for the production of secondary metabolites.

(b) What are bioreactors? How it operates?

13. (a) What are probiotics?. How do they work?

(b) Discuss the methodology and application of western blotting.

14. (a) Briefly explain the application of protoplast culture

(b) Write a short note on gene therapy

15. (a) What are reporter genes? Discuss its utility in transformation studies

(b) What are cosmids ? What is its application.

(5 x 2 = 10 Marks)

III. Answer the following questions in not more than 150 words:

16. (a) Explain PCR and its different forms with significance.

(b) Explain different types of restriction enzymes and target sites.

17. (a) Explain DVA finger printing and its application.

(b) Explain the technique and application of callus culture.

18. (a) Explain the role of linkers, adaptors and homopolymers in the generation of recombinant DNA.

(b) Discuss the role of Agrobacterium and its plasmid in the transformation of plants.

19. (a) Explain the microbial production and purification of industrial enzymes and organic compounds.

(b) Explain fermentation technology and its application.

20. (a) Explain the impact of biotechnology on agri.- biodiversity, medicine and

environment.

(b) What is genomic DNA library? Discuss the details about the construction of genomic library.

21. (a) What is cell suspension culture? Explain the kinetics of growth and elicitation methods related to suspension culture.

(b) What is bioremediation? Give an account of role of transgenic microbes in bioremediation.

22. (a) Explain gene targeting and its application

(b) Explain site directed mutagenesis with its significance.

(7 x 5 = 35 Marks)

IV. Answer the following questions in not more than 250 words:

23. (a) Explain different types of gene transfer technologies and its application.

(b) Explain the replication of bacterial DNA and regulation of gene expression

24. (a) Explain the application of transgenic technology in plants

(b) Explain about the application of plant tissue culture technology for the large scale production of crop plants.

(2 x 10 = 20 Marks)

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