Skip to main content

Fourth Semester M.Sc. Degree Examination, September 2019BotanySpecial Paper II - ElectiveBO 242 a: BIOTECHNOLOGY(2013 Admission onwards)

Reg. No....... 
Name.........

G-5263
Fourth Semester M.Sc. Degree Examination, September 2019
Botany
Special Paper II - Elective
BO 242 a: BIOTECHNOLOGY
(2013 Admission onwards)

Max. Marks: 75


1.Answer the following questions:
1.Humulin
2.YAC
3. Cybrids
4.Hybridomas
5.IPR
6.Gene therapy
7.C DNA library
8.AFLP
9. Hairy root culture
10. Somacional variation
(10 x 1=10 Marks)

II.Answer the following questions in not more than 50 words:

11. (a) What are immobilized enzymes? What is its advantage?
OR
(b) Write a short note on molecular farming.

12. (a) Give an account of bioprocess technology for the production of secondary metabolites.
OR
(b) What are bioreactors? How it operates?

13. (a) What are probiotics?. How do they work?
OR
(b) Discuss the methodology and application of western blotting.


14. (a) Briefly explain the application of protoplast culture
OR
(b) Write a short note on gene therapy


15. (a) What are reporter genes? Discuss its utility in transformation studies
OR
(b) What are cosmids ? What is its application.

(5 x 2 = 10 Marks)
III. Answer the following questions in not more than 150 words:

16. (a) Explain PCR and its different forms with significance.
OR

(b) Explain different types of restriction enzymes and target sites.

17. (a) Explain DVA finger printing and its application.
OR
(b) Explain the technique and application of callus culture.

18. (a) Explain the role of linkers, adaptors and homopolymers in the generation of recombinant DNA.
OR
(b) Discuss the role of Agrobacterium and its plasmid in the transformation of plants.

19. (a) Explain the microbial production and purification of industrial enzymes and organic compounds.
OR
(b) Explain fermentation technology and its application.

20. (a) Explain the impact of biotechnology on agri.- biodiversity, medicine and
environment.
OR
(b) What is genomic DNA library? Discuss the details about the construction of genomic library.
21. (a) What is cell suspension culture? Explain the kinetics of growth and elicitation methods related to suspension culture.
OR
(b) What is bioremediation? Give an account of role of transgenic microbes in bioremediation.
22. (a) Explain gene targeting and its application
OR
(b) Explain site directed mutagenesis with its significance.

(7 x 5 = 35 Marks)


IV. Answer the following questions in not more than 250 words:

23. (a) Explain different types of gene transfer technologies and its application.
OR
(b) Explain the replication of bacterial DNA and regulation of gene expression

24. (a) Explain the application of transgenic technology in plants
OR
(b) Explain about the application of plant tissue culture technology for the large scale production of crop plants.


(2 x 10 = 20 Marks)




Comments

Post a Comment

Popular Posts

Microbial Production of PharmaceuticalsSomatostatin, Humulin and Interferons

Microbial Production of Pharmaceuticals Somatostatin, Humulin and Interferons 1. Introduction Advances in recombinant DNA technology have enabled microorganisms to produce human therapeutic proteins safely, economically and in large quantities. Microbial systems such as Escherichia coli and yeast (Saccharomyces cerevisiae) are widely used for the production of pharmaceuticals that were earlier isolated from human or animal tissues. Important microbial-derived pharmaceuticals include somatostatin, human insulin (Humulin) and interferons. 2. Advantages of Microbial Production of Pharmaceuticals High yield and rapid production Cost-effective and scalable Free from animal pathogens Consistent product quality Easy genetic manipulation 3. General Steps in Microbial Production of Recombinant Pharmaceuticals Isolation of target gene Construction of recombinant DNA Insertion into suitable vector Transformation into host microorganism Expression of protein Downstream processing and purification ...
Post a Comment
Read more

••CLASSIFICATION OF ALGAE - FRITSCH

      MODULE -1       PHYCOLOGY  CLASSIFICATION OF ALGAE - FRITSCH  ❖F.E. Fritsch (1935, 1945) in his book“The Structure and  Reproduction of the Algae”proposed a system of classification of  algae. He treated algae giving rank of division and divided it into 11  classes. His classification of algae is mainly based upon characters of  pigments, flagella and reserve food material.     Classification of Fritsch was based on the following criteria o Pigmentation. o Types of flagella  o Assimilatory products  o Thallus structure  o Method of reproduction          Fritsch divided algae into the following 11 classes  1. Chlorophyceae  2. Xanthophyceae  3. Chrysophyceae  4. Bacillariophyceae  5. Cryptophyceae  6. Dinophyceae  7. Chloromonadineae  8. Euglenineae    9. Phaeophyceae  10. Rhodophyceae  11. Myxophyce...
Post a Comment
Read more

❃HPLC – High Performance Liquid Chromatography

HPLC – High Performance Liquid Chromatography ┏━━━━━ •❃°•°❀°•°❃•━━━━•━━━┓  1. Introduction High Performance Liquid Chromatography (HPLC) is an advanced analytical technique used for the separation, identification, and quantification of components present in a mixture. It is based on the differential distribution of analytes between a stationary phase and a liquid mobile phase under high pressure. HPLC is widely used in biochemistry, biotechnology, pharmaceuticals, food analysis, environmental studies, and clinical diagnostics. 2. Principle of HPLC The principle of HPLC is based on partition, adsorption, ion-exchange, or size-exclusion mechanisms, depending on the type of column used. A liquid mobile phase is pumped at high pressure through a column packed with fine stationary phase particles Sample components interact differently with the stationary phase Components with stronger interaction elute slower Components with weaker interaction elute faster Separated components are detec...
Post a Comment
Read more

Intellectual Property Rights (IPR) – Detailed Notes

Intellectual Property Rights (IPR) – Detailed Notes 1. Introduction Intellectual Property Rights (IPR) are legal rights granted to creators and inventors over their creations or inventions. They protect innovation and creativity, providing the owner exclusive rights to use, sell, or license their creation. IPR encourages research, development, and economic growth by rewarding creativity. 2. Importance of IPR Protects inventions, designs, and creative work. Prevents unauthorized use, copying, or commercialization. Encourages innovation and research. Provides financial benefits to inventors through licensing or royalties. Supports economic growth and competitiveness. Safeguards traditional knowledge and biodiversity. 3. Types of Intellectual Property Rights A. Patents Definition: Exclusive right granted to an inventor for a new invention for a limited period (usually 20 years). Requirements: Novelty – must be new and not published. Inventive step – non-obvious to someone skilled in the f...
Post a Comment
Read more

Exploitation of Somaclonal and Gametoclonal Variations for Plant Improvement

Exploitation of Somaclonal and Gametoclonal Variations for Plant Improvement  1. Introduction Plant tissue culture often induces genetic and epigenetic variations among regenerated plants. These variations, when stable and heritable, can be exploited as a source of novel traits for crop improvement. Somaclonal variation: Variation arising in plants regenerated from somatic cells cultured in vitro. Gametoclonal variation: Variation arising in plants regenerated from gametic cells (anther, pollen, ovule culture). Both provide additional genetic variability beyond conventional breeding. 2. Somaclonal Variation 2.1 Definition Somaclonal variation refers to genetic variation observed among plants regenerated from somatic tissue cultures, such as callus, suspension cultures, or explants. Term coined by Larkin and Scowcroft (1981). 2.2 Sources of Somaclonal Variation Chromosomal changes Aneuploidy Polyploidy Chromosome rearrangements Gene mutations Point mutations Insertions and deletions...
Post a Comment
Read more

SCAR (Sequence Characterized Amplified Region) Markers

SCAR (Sequence Characterized Amplified Region) Markers   Introduction SCAR markers are PCR-based DNA markers derived from RAPD, AFLP, or other random markers. Developed by Paran and Michelmore in 1993 to convert dominant, less reproducible markers into specific, reproducible, co-dominant markers. SCAR markers are locus-specific, reproducible, and sequence-characterized, making them ideal for marker-assisted selection (MAS). Principle SCAR markers are designed based on known DNA sequences obtained from cloned RAPD/AFLP fragments. Specific primers (18–24 bp) are synthesized to amplify a single, defined locus. The PCR amplification of this region generates a distinct band, which is highly reproducible and can distinguish homozygotes from heterozygotes if designed as co-dominant. Key idea: Random marker (e.g., RAPD) → Cloning & sequencing → Design specific primers → PCR → SCAR marker Materials Required Genomic DNA from the organism Specific primers (18–24 bp) designed from sequence...
Post a Comment
Read more

❥NORTHERN BLOTTING

NORTHERN BLOTTING – 30 MARK DETAILED NOTES  π“†ž❥ π“†ž❥ π“†ž❥ π“†ž❥ π“†ž❥ π“†ž ❥ π“†ž❥ π“†ž❥  Northern blotting is a molecular biology technique used to detect specific RNA molecules in a complex mixture. It provides information about gene expression, RNA size, and transcript abundance by hybridizing RNA with a labeled complementary DNA or RNA probe. πŸ“Œ Named by analogy to Southern blotting (DNA detection). 2. Principle The principle of Northern blotting is based on: Separation of RNA molecules by size using denaturing agarose gel electrophoresis Transfer (blotting) of separated RNA onto a nylon or nitrocellulose membrane Hybridization of membrane-bound RNA with a labeled complementary probe Detection of RNA–probe hybrids by autoradiography or chemiluminescence ✔ Only RNA sequences complementary to the probe will be detected. 3. Types of RNA Analyzed mRNA (most common) rRNA tRNA miRNA and siRNA (with modified protocols) 4. Requirements / Materials Total RNA or poly(A)+ RNA Denaturing agarose ...
Post a Comment
Read more

𓆉 INDEX PAGE -NOTETHEPOINT43

INDEX PAGE   MAIN    CONTENT 1.   HSST BOTANY SYLLABUS, DETAILED NOTES, MCQ 2.  SET GENERAL PAPER SYLLABUS, DETAILED NOTES, 50MCQ 3.  SET BOTANY SYLLABUS, DETAILED NOTES, MCQ 4. MSC BOTANY THIRD SEMESTER SYLLABUS, NOTES (KERALA UNIVERSITY ) 5. MSC BOTANY THIRD SEMESTER QUESTION PAPER (KERALA UNIVERSITY ) 6. MSC BOTANY FOURTH SEMESTER SYLLABUS &NOTES (KERALA UNIVERSITY ) 7. FOURTH SEMESTER MSC BOTANY PREVIOUS QUESTION PAPER  (KERALA UNIVERSITY )
Post a Comment
Read more

Information retrieval from databases - search concepts, Tools for searching, homology searching, finding Domain and Functional site homologies

Information retrieval from databases - search concepts, Tools for searching, homology searching, finding Domain and Functional site homologies Information Retrieval from Databases 1. Introduction Information retrieval in bioinformatics refers to the process of extracting relevant biological data (DNA, RNA, protein sequences, structures, or functional information) from databases. Aim : Identify sequences, functions, or structural features for analysis, comparison, and annotation. Databases can be primary (raw sequence data) or secondary/derived (annotated, processed data). 2. Search Concepts in Biological Databases 2.1 Types of Searches Exact Match Search Returns results only if the query exactly matches database entries. Useful for known accession numbers or IDs. Pattern/Keyword Search Searches based on specific motifs, keywords, or annotations. Example: “kinase domain,” “signal peptide.” Similarity/Homology Search Detects sequences similar to the query based on sequence alignment. Use...
Post a Comment
Read more